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. 2020 Jul 17;11:3590. doi: 10.1038/s41467-020-17349-4

Fig. 1. Cryo-EM structures of LACV-L FL.

Fig. 1

a Schematic representation of LACV-L FL domain structure. b Two views of LACV-L FL cryo-EM map at pre-initiation stage. A composite cryo-EM map was assembled from individual maps of the (i) LACV-L core, endonuclease and mid domain, (ii) mid domain and CBD, and (iii) mid domain and ZBD. Domains and RNAs are indicated with arrows and colored as in a. c Cartoon representation of semi-transparent LACV-L FL cryo-EM structure at elongation-mimicking stage rotated of 90° compared to b. Close-up views of the Coulomb-potentials and models of all RNAs visible in the elongation-mimicking map. The position of the four tunnels are shown and numbered from 1 to 4. The 5′ end vRNA (1–10) in its “5′ end stem-loop loop pocket” is displayed in yellow, the 3′ vRNA (1–16) in its “3′ end pre-initiation pocket” is shown in cyan, the 5′ vRNA (9–16) that hybridizes with the 3′ vRNA (1–16) is colored in light gray. The RNA that mimics the template and product are shown in yellow and cyan, respectively. The sequence and secondary structures of nucleic acid moieties in each complex are displayed.