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. 2020 Jul 17;11:3590. doi: 10.1038/s41467-020-17349-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a LACV-L CBD atomic model. Secondary structures are shown. The fold conserved with other sNSV CBD is shown in red and yellow. LACV CBD insertion is shown in orange. The missing loop comprising the active site is shown as a dotted line. b Multiple alignment of six Peribunyaviridae CBD: La Crosse virus (LACV), Bunyamwera virus (BUNYW), Schmallenberg (SVBVH), Macaua virus (MCAV), Wolkberg virus (WBV), and Oya virus. Secondary structures of LACV-L CBD are shown and colored as in a. Missing residues of LACV-L CBD are presented as dotted lines. Conserved residues of Peribunyaviridae CBD active site motif (WNxWQxxR) are shown as orange stars. c Cryo-EM 3D classes corresponding to LACV-L CBD extreme position 1 and 2 are superimposed. CBD movement is compared to LACV-L core and endonuclease that adopt stable positions. Their CBD are, respectively, shown in blue and purple. The endonuclease domain is shown in green. LACV-L core is shown in gray. d Overview and close-up view of the CBD/endonuclease domain interactions in the extreme position 1. Interacting residues are identified and labeled. CBD coloring is the same as in a. e Overview of the CBD/endonuclease domain interaction in the extreme position 2. Interacting residues are identified and labeled. CBD side chain positions remain however hypothetical due to the CBD EM map resolution in extreme position 2.