a Model for metabolite transfer in bacteriomes. Lactate released as an end product of aerobic glycolysis from Sulcia bacteriomes is imported by Zinderia bacteriomes and oxidized to produce energy. Essential amino acids produced in each bacteriome are listed in gray boxes and energy costs (μmol ATP per μmol amino acid synthesized) for the synthesis of each amino acid are provided in parentheses. Energy costs data obtained from ref. [9]. Reaction fluxes (solid arrows) and transport fluxes (dashed arrows) are shown. Arrow thickness illustrate the magnitude of flux size, thicker arrows represent higher fluxes. GLUT glucose transporter, LDH lactate dehydrogenase, MCT monocarboxylate transporter, TCA tricarboxylic acid, OXPHOS oxidative phosphorylation, ATP adenosine triphosphate. In silico glucose and lactate choice assay for (b) Sulcia bacteriomes and (c) Zinderia bacteriomes. d In silico predictions of ATP synthase flux in Zinderia bacteriomes when provided with glucose, a mixture of glucose and lactate or lactate as carbon sources.