Fig. 8. Hepatic TFEB induction lowers hepatic and plasma cholesterol in WD-fed mice.

a–d Male 10-week old C57BL/6J mice were injected Ad-Null or Ad-TFEB at a dose of 5 × 10⁸ p.f.u. per mouse via tail vein. Mice were fed chow diet for one week and then either fed chow diet or challenged with WD for one additional week. (n = 5 mice per group). Liver total cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE) (a), plasma total cholesterol (b), gallbladder (GB) total cholesterol (c), and tissue bile acids (d) were measured. Bile acid pool is the sum of total bile acids in liver, gallbladder and small intestine with luminal content. All results were expressed as mean ± SEM. Two-way ANOVA and Tukey post hoc were used for a–c; Two-sided Student’s t-test was used for d. Source data for a–d are provided as a Source Data file.