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. 2020 Jul 17;11:3624. doi: 10.1038/s41467-020-17177-6

Fig. 1. Viral infection reduces MG53 expression in macrophages.

Fig. 1

a PMA-differentiated THP1 cells express endogenous MG53. MG53 protein in THP1 cells could be immunoprecipitated with anti-MG53 antibody. Mouse skeletal muscle (0.2 µg) was loaded as a positive control. THP1 protein lysate (20 µg) was loaded as input (data representative of three independent experiments). b De-identified primary human blood Monocyte-Derived Macrophages (hMDMs) also express MG53 protein. Murine skeletal muscle (0.5 µg) and 15 µg hMDM protein lysates (Donor 1/D1 and Donor 2/D2) were loaded for western blotting and MG53 immuno-detection (data representative of three independent experiments). Macrophage MG53 levels are lower than that seen in murine skeletal muscle (see also Supplementary Fig. S1). c Differentiated THP1 cells were infected with SeV (MOI 5) for 24 or 48 h. THP1 protein lysate (20 µg) was loaded for western blot and immune-probed for MG53 and GAPDH expression (data representative of three independent experiments conducted at 24 h and two independent experiments at 48 h). Infected cells had over 50% reduction in MG53 expression relative to noninfected controls. d Quantification of reduction in MG53 protein expression following SeV infection (data show three separate experiments conducted at 24 h and two separate experiments at 48 h, each experiment contained three independent infection replicates; mean ± SD; ***p = 0.0005, ****p < 0.0001; two-sided one-sample t tests).