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. 2020 Jul 17;11:3624. doi: 10.1038/s41467-020-17177-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Protein lysates (10 µg) from the indicated THP1 lines were loaded for western blot to assess p65 phosphorylation. Western blotting showed a ~2-fold increase in basal p65 phosphorylation in shMG53-THP1 cells compared with sh-control (n of four independent experiments; mean ± SE; **p = 0.0083; two-sided paired t test). b Cellular localization of p65 was assessed by immunofluorescence using p65 antibody. Nuclear localization of p65 is significantly increased in sh-MG53 THP1 cells compared to sh-control (n = 119 THP1, 119 sh-MG53, and 65 sh-control cells examined over two independent experiments; mean ± SD; n.s. means nonsignificant p = 0.6300, **p = 0.0013, ****p < 0.0001; one-way ANOVA with Tukey’s multiple comparison test).