Table 2.
Effect of treatment, invasion status, climatic regime and number of siblings on post-metamorphic traits of Italian agile frogs: results of linear mixed models (two-sided F tests without multiple test corrections) including development time as fixed factor in the model (a–c) and results excluding development time from the model (d–f).
Post-metamorphic trait | Fixed effects | F | df | P | R2m | R2c |
---|---|---|---|---|---|---|
Analyses including development time as covariate | ||||||
(a) Body size | Climatic regime | 5.189 | 1, 99.5 | 0.017 | ||
Invasion status | 9.79 | 1, 103.0 | 0.002 | |||
Crayfish exposure | 7.42 | 1, 9.2 | 0.023 | 0.35 | 0.35 | |
No. of siblings | 7.44 | 1, 102.9 | 0.007 | |||
Development time | 50.38 | 1, 100.5 | <0.001 | |||
(b) Tibiofibula length | Climatic regime | 1. 77 | 1, 100.6 | 0.187 | 0.33 | 0.33 |
Invasion status | 12. 71 | 1, 104.2 | <0.001 | |||
Crayfish exposure | 12.06 | 1, 9.4 | 0.007 | |||
No. of siblings | 10.29 | 1, 104.4 | 0.002 | |||
Development time | 47.84 | 1, 101.6 | <0.001 | |||
(c) Max jumping distance | Climatic regime | 1.39 | 1, 101.5 | 0.242 | ||
Invasion status | 4.22 | 1, 107.7 | 0.042 | |||
Crayfish exposure | 10.20 | 1, 7.2 | 0.015 | 0.27 | 0.30 | |
No. of siblings | 12.11 | 1, 106.8 | <0.001 | |||
Development time | 35.034 | 1, 102.4 | <0.001 | |||
Analyses not including development time as covariate | ||||||
(d) Body size | Climatic regime | 2. 94 | 1, 104.0 | 0.089 | ||
Invasion status | 0.77 | 1, 104.0 | 0.382 | |||
Crayfish exposure | 0.12 | 1, 104.0 | 0.730 | 0.03 | 0.03 | |
No. of siblings | 0.11 | 1, 104.0 | 0.740 | |||
(e) Tibiofibula length | Climatic regime | 0.66 | 1, 105.0 | 0.417 | ||
Invasion status | 1.55 | 1, 105.0 | 0.216 | |||
Crayfish exposure | 0.90 | 1, 105.0 | 0.344 | 0.02 | 0.02 | |
No. of siblings | 0.65 | 1, 105.0 | 0.420 | |||
(f) Max jumping distance | Climatic regime | 0.71 | 1, 101.3 | 0.402 | ||
Invasion status | 0.08 | 1, 108.0 | 0.782 | |||
Crayfish exposure | 2.07 | 1, 5.8 | 0.202 | 0.04 | 0.06 | |
No. of siblings | 1.82 | 1, 108.0 | 0.180 |
For all tests we report both marginal and conditional determination coefficients (R2m and R2m, respectively74). Significant effects are in bold. n = 110 tadpoles.