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. 2020 Jul 17;10:11921. doi: 10.1038/s41598-020-68629-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MCPH1 overexpression reduces centrosome amplification in MCPH1-deficient cancer cell lines. (a) Western blots demonstrating MCPH1 levels in cell lines without MCPH1 deletion (HeLa, MCF10A, and RPE), heterozygous MCPH1 deletion (CAL-51, MDA-MB-231, MDA-MB-453, and MDA-MB-468), and homozygous MCPH1 deletion (PC3). Indications are made for molecular weight markers. (b) Immunofluorescence images of the cell lines from panel (a) transfected with GFP-MCPH1 or GFP control. (c) Quantification of centrioles in the conditions shown in panel (b). Each dot represents a single cell, color coded by each independent experiment, with mean values for each experiment indicated by larger, outlined dots. Bars represent mean ± SEM from 3 independent experiments. (d) Quantification of CA, defined as the percentage of cells with greater than 4 centrin foci. Each dot represents a single experiment, and bars represent means ± SEM. Vertical dotted lines are shown to separate cell lines based on MCPH1 status.