Figure S4. Characterization of rescued P19 cell lines.

(A) Western blot analysis of the expression levels of γ1-COP and γ2-COP, and α-tubulin as a loading control in pluripotent P19 WT, Copg1^−/−, PB-Copg1 (Copg1^−/− cells rescued with a PiggyBac transposon vector), Copg1^−/− + Copg1-GFP (Copg1^−/− cells rescued with a bacterial artificial chromosome), and Copg2^−/−, PB-Copg2 cell lysates. The asterisk (*) marks a nonspecific signal. The arrow head indicates γ1-COP-GFP in lane 4. (A, B) Same as in (A) for P19 WT, Copg1^−/− + Copg1-GFP, and Copg1^−/−, Copg2^−/− + Copg1-GFP cells. (C) Growth curves obtained from a real-time proliferation assay in which the occupied area (% confluence) by P19 WT, Copg1^−/−, and Copg1^−/− + Copg1-GFP cells was monitored over 72 h. Curves were generated with the IncuCyte software (n = 5, error bars are SEM).

Source data are available for this figure.