Figure 2.
In vivo pathogenicity assay of the vegetative and reproductive stage. (a) T6SS deletion mutants were inoculated with 108 cfu/ml to assess the virulence of the T6SS cluster in the xylem of rice stem (Oryza sativa) at the vegetative stage. The experiments were conducted by three replicates (n = 3). (b) Bacterial suspensions were inoculated into rice panicles at the reproductive stage to assess the virulence of ΔtssD4, ΔtssD5, ΔtssD45, ΔtssD4‐C, ΔtssD5‐C, and ΔtssD45‐C. Representative of four replicates. (c) Disease severity on the rice panicles was calculated on a scale of 0 to 5 after inoculating the bacterial suspension. The data are presented as the mean ± SD of four replicates (n = 4). Mean values followed by the same letters are not significantly different according to Tukey's HSD test (*p < .05, **p < .01, ***p < .001). Disease symptoms at 8 days post‐inoculation. Distilled water (D.W.) was used as the negative control