Figure 8.

The aurofusarin cluster C13 is up‐regulated in runner hyphae (RH) and responsible for toxicity to bacteria and fungi. (a) The aurofusarin gene cluster C13 (from left to right: FGSG_02320 – FGSG_02330) is specifically up‐regulated in RH compared to infection cushions (IC) or mycelium grown in complete medium (MY). No significant difference in gene expression of C13 cluster was found between IC and MY. Differential expression of genes is given by log₂ FC(FPKM + 1) values. Asterisks indicate significant up‐regulation for log₂ FC(FPKM + 1) ≥ 2 or down‐regulation log₂ FC(FPKM + 1) ≤ −2. (b) Bioactivity assay using extracts of wild‐type (WT) and aurofusarin‐deficient mutant ∆pks12. For bacteria and yeasts, OD₅₉₅ was measured after 16 hr of growth in medium supplemented with either WT extracts, extracts of the aurofusarin‐deficient ∆pks12 mutant, or phosphate buffer as a control. For filamentous fungi, dry weight was calculated. The highest value of buffer controls was set to 100% of growth. For every condition and organisms tested, three biological replicates were performed