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. 2020 Jun 12;2(7):407–414. doi: 10.1002/acr2.11155

Figure 2.

Figure 2

Follistatin‐like protein 1 (FSTL1) expression regulates chondrocyte phenotype. CH‐hTERT/E6/E7 chondrocytes were transduced with lentivirus encoding FSTL1 short hairpin RNA shRNA) or scrambled shRNA (control). Chondrocyte differentiation was performed in the presence of transforming growth factor β. A, After 21 days of culture, FSTL1, COL2A1, ACAN, MMP13, COL10A1, and RUNX2 were assayed by real‐time PCR. The mean + SEM is shown (n = 3; FSTL1 *P = 0.01, COL2A1 *P = 0.01, ACAN **P = 0.028, MMP13 *P = 0.01, COL10A1 ***P = 0.0015, RUNX2 ****P = 0.0039; two‐tailed t test). B, After 7, 14, or 21 days of culture, cell lysates were assayed by Western blotting for FSTL1, MMP13, or actin.