Figure 4.

Effects of HAS on ROS levels of H₂O₂-stimulated PC12 cells. PC12 cells were treated with vitamin C (100 μM) or HAS (5, 30, and 60 μM) for 2 h, subsequently subjected to H₂O₂ (90 μM) for 4 h. The intracellular ROS level was determined by the flow cytometry (FCM) assay. The values were represented as the mean ± SD (n = 3). ^∗∗P < 0.01 vs. the control group.