Table 1.

List of Laminin^mur-Binding Protein Spots Identified From the Outer Membrane Fraction by LC-MS/MS Analysis

Protein Spot No.	Protein Description and Function	Gene	GenBank Accession Numbera	Molecular Weight (Da)b	pIb	Score	Sequence Coverage (%)
L1c	Elongation factor thermal unstable (EF-Tu)	tufA d	EDJ92442	43297.31	5.26	113	25
L2c	Protein D, glycerophosphoryl diester phosphodiesterase (PD)	hpd e	EDJ92229	41873.98	6.51	153	29
L3c	l-lactate dehydrogenase (LDH)	ldh e	EDJ92617	41873.32	6.55	221	36
L4	Lipoprotein E, NADP phosphatase (P4)	hel	EDJ92235	30501.34	8.98	316	29
L5c	Peptidoglycan-associated outer membrane lipoprotein P6 (P6)	hp6 e	EDJ93375	16107.91	6.09	210	38
L6c	30S ribosomal protein S6 (Rib30s)	rpaF	EDJ92478	14484.32	5.46	192	58

Abbreviations: LC-MS/MS, liquid chromatography-tandem mass spectrometry; NTHi, nontypeable Haemophilus influenzae; ORF, open reading frame.

^aAccession number indicated is based upon the NTHi 3655 annotated genome database.

^bMolecular weight and pI of each protein is predicted using Protparam tool (https://web.expasy.org/protparam/).

^cFull-length ORFs were cloned into pET26(b)+ for expression of His-tagged recombinant proteins in Escherichia coli BL21 (DE3) followed by purification.

^dEF-Tu is encoded by 2 alleles tufA (EDJ92442) and tufb (EDJ92095) in NTHi 3655.

^eAlternative gene names for hpd, ldh, and hp6 are glpQ, lctD, and pal, respectively.