Fig. 2.

(A) Confocal images of DR5:Venus-NLS under control conditions and during BLM recovery for indicated times. hpr, hours postrecovery. Images represent maximum intensity projections from 10 slices acquired at 1.5-µm intervals. Dashed red lines indicate the outline of the root and the region containing the dead cells, as inferred from propidium iodide staining (SI Appendix, Fig. S2A). (B and C) Confocal images of a R2D2 ratiometric auxin reporter line grown under control conditions (B) and after BLM treatment (C). Cartoons are representative images reconstructed from average R/G ratios calculated from 12 images. A higher red/green ratio corresponds to higher auxin levels. (Scale bars in A and B: 50 μm.) VASC, vasculature; EN, endodermis; CO, cortex; EP, epidermis. (D) BLM recovery percentages of wild-type, 35S:ERF115-SRDX, tir1-1, and tir1-1afb2-3 mutants. ***P < 0.001. (E) Side-by-side comparison of average fold changes in auxin concentrations as observed in vivo (R2D2) and predicted by the in silico model (MODEL). Error bars represent maximal errors calculated as described in Materials and Methods. Fold changes (BLM/control) in R/G ratios were obtained from R2D2 images according to the cell position in the respective tissues. Ratios from all cells within cell positions 1 to 5, 6 to 10, and 11 to 15 were averaged and plotted as three data points. *P < 0.05; **P < 0.01; ***P < 0.001.