Table 1.

Virus replication in the respiratory tract of hamsters following rechallenge

Primary infection dose	Animal ID	Antibody endpoint titer in serum*	Neutralizing antibody titer in serum†	Virus titers (log10 PFU/g) of animals infected with SARS-CoV-2‡
				Nasal turbinate	Trachea	Lung
High dose (105.6 PFU)	#1	40,960	1,280	—	—	—
	#2	40,960	640	—	—	—
	#3	40,960	1,280	—	—	—
Low dose (103 PFU)	#4	40,960	640	—	—	—
	#5	40,960	640	—	—	—
	#6	40,960	1,280	—	—	—
Mock (PBS)	#7	<10	<20	5.5	3.3	7.1
	#8	<10	<20	5.4	4.7	7.4
	#9	<10	<20	5.3	3.5	7.4

Syrian hamsters were inoculated with 10^5.6 PFU (in 110 μL) of UT-NCGM02 via a combination of the intranasal (100 μL) and ocular (10 μL) routes on day 20 after the primary infection. Three Syrian hamsters per group were killed on day 4 after rechallenge for virus titration.

^*Viral antibody endpoint titers against the receptor-binding domain expressed as the reciprocal of the highest dilution with an optical density at 490 nm (OD₄₉₀) cutoff value >0.15; sera were collected on day 19 after the primary infection.

^†Viral neutralization titers against inoculated virus; sera were collected on day 19 after the primary infection.

^‡A dash denotes that virus was not detected.