Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 29;117(28):16465–16474. doi: 10.1073/pnas.2003022117

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published under the PNAS license.

PMC Copyright notice

Fig. 4. — Langerhans cells promote the commensal-derived immune responses. (A) Absolute number of live IL-17A-producing CD4⁺Foxp3^neg T cells (CD4⁺IL-17A⁺) and IL-17A-producing CD8⁺ T cells (CD8⁺IL-17A⁺) after C. albicans association in the ear pinnae from Lan-DTA and wild-type littermate control (WT) SPF mice. (B) Percentage of ear thickness change relative to baseline at day 0 from IMQ- and C. albicans/IMQ (C. alb/IMQ)-treated Lan-DTA SPF mice. (C) Absolute numbers of live neutrophils in the ear pinnae from IMQ- and C. albicans/IMQ (C. alb/IMQ)-treated Lan-DTA, Batf3^−/−, and Irf4^fl/fl Itgax^cre+ SPF mice. (D and E) Percentage of ear thickness change relative to baseline at day 0 from IMQ- and C. albicans/IMQ (C. alb/IMQ)-treated Batf3^−/− (D) and Irf4^fl/fl Itgax^cre+ (E) SPF mice. (F) Absolute numbers of live IL-17A-producing CD4⁺Foxp3^neg (CD4⁺IL-17A⁺) T cells from the ear pinnae of IMQ- and C. albicans/IMQ (C. alb/IMQ)-treated Lan-DTA, Batf3^−/−, and Irf4^fl/fl Itgax^cre+ SPF mice. (G) Absolute numbers of LCs, CD103⁺ DCs, and CD11b⁺ DCs in the ear pinnae from Ctrl, C. alb-, IMQ-, or C. alb/IMQ-treated SPF mice. (H) Dectin-1 mean fluorescence intensity (MFI) of DC subsets in the ear pinnae from Ctrl, C. alb-, IMQ-, or C. alb/IMQ-treated SPF mice. (I and J) Percentage of ear thickness change relative to baseline at day 0 (Left) and absolute numbers (Right) of live IL-17A-producing CD4⁺Foxp3^neg (CD4⁺IL-17A⁺) T cells in the ear pinnae from IMQ- and C. alb/IMQ-treated Clec7a^−/− (I) or Card9^−/− (J) SPF mice. Cytokine production shown in this figure was analyzed following in vitro restimulation of the cells with PMA and ionomycin in the presence of brefeldin A. Results are representative of two to three independent experiments with the exception of results shown for Lan-DTA mice in F, which are a compilation of results from two independent experiments. *P < 0.05, **P < 0.01; N.S., not significant.