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. 2020 Jun 29;117(28):16500–16508. doi: 10.1073/pnas.2000648117

Fig. 3.

Fig. 3.

Paracrine coculture with fibroblasts results in sustained MTOR signaling in tumor cells. (A–D) Pathway score changes in EFM192, HCC202, BT474, and HCC1954 induced by lapatinib (0.1 μM) treatment in monoculture and coculture conditions. Values are average log2-transformed ratios of each sample normalized to the dimethyl sulfoxide (DMSO) monoculture control in each cell line for at least two biological replicates. Error bars are SEM. (D) Heatmap of protein expression changes for MTOR/PI3K/Apoptotic pathway members. Red color indicates a protein increase, while blue indicates protein decrease for the average log2-transformed ratios. (E) Apoptosis pathway scores visualized in the same way as A–C. (F) Analysis of phospho-HER2 (Tyr1248), phospho-EGFR (Tyr1068), phospho-AKT (Ser473), and phospho-S6 (Ser235/236) protein expression changes in direct EFM192-AR22 cocultures using immunofluorescence. Cells were dosed with 0.1 μM of lapatinib for 48 h. Data are representative of two biological replicates.