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Effect of IL-38 on secretion of CXCL8 from neonatal human microglia stimulated by neurotensin. Human microglia cells were pretreated with IL-38 (aa3-152, 1–100 ng/mL) for 24 h and then were stimulated with NT (10 nM) for 24 h to determine the secretion of CXCL8 by specific ELISAs. All conditions were performed in triplicates for each dataset and repeated twice (n = 2).
