Fig. 2.

icu11-3 affects global histone methylation and DNA methylation at the pericentromeric cen180 repeats. (A) The icu11-3 mutant shows elevation of H3K36me3 and H3K4 methylation, but no change in H3K27me3 compared with WT C12b. Histones were prepared from a nuclear extract from 14-d-old seedlings and serially diluted; Western blots are shown with total histone H3 from the same extraction as loading controls. (B) Southern blot analysis of methylation at the pericentromeric cen180 repeat region. Genomic DNA digested with the methylation-insensitive restriction enzyme MspI (control) and methylation-sensitive enzyme HpaII indicates slightly reduced DNA methylation over centromeric repeats in icu11-3.