Table 1.
Protein | 35S::GFP | gICU11-GFP icu11-3 | icu11-3 | gICU11-HA icu11-3 | Col-0 | EMF1-3XFLAG emf1 | 35S::GFP | 35S:GFP-CLF clf-50 | gSWN-GFP | |
Core PRC2 | ICU11 | 0–0 | 13–19 | 0–0 | 12–11 | 0–0 | 11–20 | 0–0 | 13–2 | 5–5 |
EMF2 | 0–0 | 11–4 | 0–0 | 29–0 | 0–0 | 2–22 | 0–0 | 33–18 | 4–36 | |
MSI1 | 0–0 | 12–6 | 0–0 | 25–2 | 3–2 | 6–18 | 2–0 | 22–18 | 6–26 | |
FIE | 0–0 | 10–1 | 0–0 | 18–0 | 0–0 | 5–17 | 2–0 | 23–22 | 6–25 | |
SWN | 0–0 | 10–0 | 0–0 | 27–0 | 0–0 | 0–26 | 0–0 | 11–0 | 21–68 | |
CLF | 0–0 | 7–0 | 0–0 | 26–0 | 0–0 | 3–17 | 9–0 | 75–46 | 8–4 | |
Accessory | EMF1 | 0–0 | 5–3 | 0–0 | 26–2 | 0–0 | 6–40 | 0–0 | 46–17 | 2–37 |
LHP1 | 0–0 | 2–1 | 0–0 | 11–0 | 0–0 | 2–14 | 0–0 | 22–8 | 3–18 | |
TRB1 | 0–0 | 5–9 | 0–0 | 12–2 | 0–0 | 0–1 | 0–0 | 0–0 | 0–0 | |
TRB2 | 0–0 | 9–5 | 0–0 | 15–1 | 0–0 | 1–11 | 0–0 | 11–3 | 2–10 | |
TRB3 | 0–0 | 9–6 | 0–0 | 15–3 | 0–0 | 3–12 | 0–0 | 9–4 | 2–7 | |
VRN5 | 0–0 | 0–0 | 0–0 | 0–0 | 0–0 | 0–0 | 0–0 | 24–13 | 0–24 | |
VEL1 | 0–0 | 0–0 | 0–0 | 0–0 | 0–0 | 0–0 | 0–0 | 41–28 | 0–46 | |
ALL PEPTIDES | 787–1817 | 1286–7239 | 2944–3524 | 5481–6267 | 11483–16737 | 11170–13095 | 6705–2489 | 7376–7714 | 5127–6855 |
The numbers indicate uniquely identified peptides from each protein found by mass spectrometry in two independent experiments. The total number of peptides identified in each experiment is also indicated at the bottom of the columns. PRC2 core components are shown at the top, with accessory components below. Columns are paired with control on left and immunoprecipitated to the right. For example, 35S:GFP samples in column 1 were the control for the ICU11-GFP samples in column2 in the gICU11-GFP analysis. 35S:GFP-CLF clf50 and gSWN-GFP share the 35S::GFP control. The full list of proteins identified is presented as an excel sheet in Dataset S1.