Fig. 2.

Solution-state NMR on monomeric CRES. (A) Two-dimensional ¹⁵N,¹H HSQC spectrum of 1.4 mM CRES in 25 mM MES, 250 mM NaCl, 1 mM EDTA, pH 6 at 25 °C. Resonance assignments are given as the residue number and amino acid single letter code. (B) Secondary structure propensity of solution-state CRES derived from backbone chemical shifts and TALOS-N. The secondary structure observed in the crystal structure is diagrammed above. (C) Comparison of the average b-factors from the four CRES monomers in the crystallographic asymmetric unit (green points) and the predicted random coil index (RCI) order parameter (S²) (blue points), which were also calculated from the backbone chemical shifts using TALOS-N. The SDs of the crystallographic b-factors of the four monomers are given as gray error bars.