Table 1.

Data collection and refinement statistics

	${\text{tRNA}}_{\text{GGC}}^{\text{Ala}}$ cognate codon	${\text{tRNA}}_{\text{GGC}}^{\text{Ala}}$ near-cognate codon	${\text{tRNA}}_{\text{GGC}}^{\text{Ala}}$ UA cognate codon	${\text{tRNA}}_{\text{GGC}}^{\text{Ala}}$ UA near-cognate codon
PDB ID	6OF6	6OJ2	6ORD	6OPE
Data collection
Wavelength (Å)	1.0000	1.0000	0.9792	0.9791
Space group	P212121	P212121	P212121	P212121
Cell dimensions
a, b, c (Å)	211.3, 452.3, 626.5	211.0, 453.5, 625.4	209.4, 445.8, 616.0	209.4, 449.8, 620.0
α, β, γ (degrees)	90, 90, 90	90, 90, 90	90, 90, 90	90, 90, 90
Resolution (Å)	43.9 to 3.2 (3.3 to 3.2)	50 to 3.2 (3.3 to 3.2)	49.9 to 3.1 (3.2 to 3.1)	49.6 to 3.1 (3.2 to 3.1)
Rpim (%)	7.4 (34.4)	11.0 (74.0)	12.7 (83.7)	14.1 (77.0)
I/σI	8.26 (2.04)	4.88 (0.98)	4.57 (0.71)	6.29 (1.12)
CC1/2	0.993 (0.645)	0.995 (0.329)	0.991 (0.183)	0.989 (0.387)
Completeness (%)	91.15 (91.01)	99.52 (99.92)	95.19 (91.69)	97.13 (99.13)
Redundancy	6.6 (6.0)	5.8 (5.6)	3.0 (2.2)	3.0 (3.0)
Refinement
No. reflections	889,696	971,076	981,305	1,016,419
Rwork/Rfree (%)	19.5/23.2	23.6/27.0	23.2/26.8	22.5/26.6
No. atoms
Macromolecules	297,356	297,318	290,854	294,030
Ligands	1,319	1,053	2,965	951
B-factors
Macromolecules	90.33	129.71	91.34	87.33
Ligands	56.13	130.73	60.81	59.98
rms deviations
Bond lengths (Å)	0.005	0.006	0.008	0.012
Bond angles (degrees)	0.87	1.07	0.99	1.25

Values in parentheses are for highest-resolution shell.