Fig. 1.

Observed frequency of mitochondrial and plastid SNVs in wild-type Arabidopsis tissue based on duplex sequencing. Dark triangles represent three biological replicate families of wild-type A. thaliana Col-0. Lighter circles are F3 families derived from homozygous wild-type plants that segregated out from a heterozygous parent containing one mutant copy of an RRR candidate gene. Variant frequencies are calculated as the total number of observed mismatches in mapped duplex consensus sequences divided by the total base pairs of sequence coverage.