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Estimates of heteroplasmic frequency of select SNVs using ddPCR. The F3 pool is the same msh1-CS3246 mutant mitochondrial or plastid DNA sample in which the SNV was discovered by duplex sequencing. F4 indicates plants descended from the F3 family used in duplex sequencing. Controls are F4 plants from msh1-CS3246 mutant lines other than the one in which the SNV was originally discovered.
