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. 2020 Jul 18;6(1):dvaa009. doi: 10.1093/eep/dvaa009

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© The Author(s) 2020. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2: — Reverse transcriptase-dependent BrdU incorporation occurs in zygote pronuclei. Upper panel: schematic representation of the protocol used to assess BrdU incorporation in parental pronuclei of in vitro fertilized (IVF) embryos in the presence of a DNA replication inhibitor (aphidicolin) or an RT inhibitor (abacavir). The immunofluorescence panels below show that BrdU incorporation (green) takes place in both parental pronuclei (blue), more intensely in the paternal one, independent on DNA replication, but is full inhibited in embryos exposed to the combination of aphidicolin + abacavir, indicating that is RT-dependent. Bar, 20 µm From Ref. [96].