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. Author manuscript; available in PMC: 2020 Jul 18.
Published in final edited form as: J Am Chem Soc. 2018 Dec 27;141(1):290–297. doi: 10.1021/jacs.8b09928

Figure 2. Enzymatic reconstitution of fusilassin.

Figure 2.

(A) MALDI-TOF-MS of all requisite biosynthetic components (top) shown alongside parallel reactions with individual components omitted from the reaction. (B) Relative processing of core position 1 variants from purified enzyme assays with wild-type Trp1 as the top spectrum. W1E product formation was at the limit of detection (Fig. S8). Spectra represent end-point assays after 3 h reactions. Macrolactam-containing products are blue and linear core (uncyclized) peptides are orange. Low-intensity peaks at +22 Da represent sodiated ions of either the linear core or mature lasso peptide.