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. Author manuscript; available in PMC: 2020 Jul 18.
Published in final edited form as: J Am Chem Soc. 2018 Dec 27;141(1):290–297. doi: 10.1021/jacs.8b09928

Figure 5. RRE:Leader peptidase interaction.

Figure 5.

MALDI-TOF-MS was used to monitor leader peptidase activity of FusB and a panel of GREMLIN- and structure-derived FusE variants. Variants demonstrated differing degrees of enzymatic processing, supporting our GREMLIN predictions. SGS refers to the N-terminal Ser-Gly-Ser motif remaining after TEV protease cleavage of MBP-FusA.