Table 1.
Minimal Inhibitory Concentrations and Antibiotic Resistance Genes in Methicillin-Resistant Staphylococcus pseudintermedius 8510 Strain
| Antibiotics | MICs (μg mL-1) | Interpretive category | Resistance genes from MRSP 8510 genome |
|---|---|---|---|
| Penicillin | >32a | R | blaZ |
| Oxacillin | >2 | Rc | mecA |
| Ceftaroline | 0.5 | Sd | |
| Erythromycin | >4 | R | ermB |
| Clindamycin | >2 | R | — |
| Kanamycin | 6 mmb | NA | aph(3′)-III |
| Streptomycin | 6 mmb | NA | ant(6)-Ia |
| Gentamicin | 4 | S | — |
| Tetracycline | 0.12a | S | — |
| Minocycline | ≤1 | S | — |
| Tigecycline | ≤0.12 | Se | — |
| Trimethoprim-sulfamethoxazole | >2/38 | R | dfrG |
| Chloramphenicol | 64a | R | catA |
| Ciprofloxacin | >2 | R | grlA (Ser80Ile); gyrA (Ser84Leu, Glu88Val)f |
| Vancomycin | ≤0.5 | S | — |
| Teicoplanin | ≤1 | S | — |
| Rifampicin | ≤0.5 | S | — |
| Linezolid | ≤1 | S | — |
| Daptomycin | ≤1 | S | — |
Performed by E-test (BioMerieux).
Performed by disk diffusion.
All the antibiotics were interpreted according to Staphylococcus spp. CLSI breakpoints (M100 28th ed.) except for: coxacillin (Staphylococcus pseudintermedius CLSI breakpoint), dceftaroline (Staphylococcus aureus CLSI breakpoint), etigecycline (Food and Drug Administration breakpoint).
Mutations in the quinolone-resistance determining region of the topoisomerase genes.
—, no gene was detected; CLSI, Clinical Laboratory Standards Institute; MICs, minimal inhibitory concentrations; MRSP, methicillin-resistant S. pseudintermedius; NA, not available; R, resistant; S, susceptible.