Figure 2. Expression and purification of canine SOD1.

(A) A Coomassie stained SDS-PAGE gel of purified canine SOD1. (B) Enzymatic activity of canine SOD1. The activities of Cu²⁺/Zn²⁺-bound canine SOD1 (holo-) were significantly higher than those of Cu²⁺/Zn²⁺-unbound canine SOD1 (apo-). No significant difference was found between WT, E40K and T18S canine SOD1. Each data point is mean ± SEM. (C and D) Far-UV circular dichroism spectra of (C) apo- and (D) holo-WT, E40K, and T18S canine SOD1. Each data point is mean ± SEM.