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. 2020 Jul 7;13:6709–6720. doi: 10.2147/OTT.S253151

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© 2020 Yang et al.

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The association between miR-638 and SP1 in HCC. (A and B) SP1 mRNA and protein levels in HCC and para-tumor tissues were examined via qRT-PCR and western blot. (C) The linear correlation between expression of miR-638 and SP1 in HCC samples was analyzed. (D) SP1 protein expression was detected in HCC and normal liver cells. (E) The complementary sequence between miR-638 and SP1 was predicted via starBase. (F and G) Dual-luciferase reporter and RIP assays were conducted in Huh7 cells with transfection of miR-638 mimic or NC. (H) SP1 protein expression was detected in Huh7 cells transfected with 50 or 100 nM miR-638 mimic or NC. *P<0.05.