Figure 1.

Generation of CCL19-expressing Fib and MSC. (A) Murine Fib and MSC were immortalized by transfection with SV40T-GFP vector. GFP expression in primary (p)Fib, immortalized (i)Fib, pMSC, and iMSC was examined by flow cytometry. (B) mRNA expression of iFib, iMSC, CCL19-expressing iFib (iFib/CCL19), and CCL19-expressing iMSC (iMSC/CCL19) was examined by RT-PCR. β-Actin was used as a control. (C) The levels of CCL19 in the supernatants were determined by ELISA. Data are presented as the mean±SEM. **P<0.01 by Student’s t-test. (D) CT26 colon carcinoma cells (5×10⁵) were inoculated subcutaneously into BALB/c mice with or without pMSC or iMSC. Tumor size was measured every 4 days. Data are presented as the mean±SEM. CCL19, chemokine (C-C motif) ligand 19; iFib, immortalized fibroblasts; iMSC, immortalized MSC; MSC, mesenchymal stem/stromal cells; pFib, primary fibroblasts; pMSC, primary MSC; RT, reverse transcription.