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. 2020 Jul 16;8(2):e000582. doi: 10.1136/jitc-2020-000582

Figure 6.

Figure 6

Local injection of iMSC/CCL19 augments the antitumor effect by anti-PD-L1 blockade therapy. (A) PD-L1 expression on CT26 cells that were cultured in the presence or absence of recombinant 50 ng/mL IFN-γ for 48 hours. Gray: isotype control, black dotted: PE anti-PD-L1 antibody. (B) CT26-bearing mice were i.t. injected with iMSC or iMSC/CCL19 on days 14 and 16; on day 17 after tumor inoculation, TILs were collected and stained with anti-CD3, anti-CD8α, and anti-PD-1 antibodies. Splenic cells from CT26-bearing mice were analyzed as control cells. The numbers represent percentages of PD-1+ CD8+ cells among CD3+ CD8+ T cells. (C) CT26-bearing mice were i.t. injected with iMSC/CCL19 on days 13 and 15 after tumor inoculation (arrowhead). Control IgG (200 µg) or anti-PD-L1 (200 µg) antibody were i.p. injected on days 15 and 17 (arrow). The tumor growth in individual mice is shown. (D) The mean (±SEM) tumor size. *P<0.05. **P<0.01 by two-tailed t-test. N.S., not significant. (E) Photograph of tumor tissues is shown. Scale bar=10 mm. CCL19, chemokine (C-C motif) ligand 19; IFN, interferon; iMSC, immortalized MSC; i.t., intratumoral; MSC, mesenchymal stem/stromal cells; TILs, tumor-infiltrating lymphocytes.