Figure 3.

CD8⁺ T cells producing IFNγ in draining lymph nodes (dLN) and spleens (SP) of mice bearing primary MC-38 tumors. Lymph nodes and spleens from 10 naïve animals and 10 tumor-bearing animals were collected at each time point post inoculation and disaggregated into a single-cell suspension. All cells were pooled and triplicate aliquots (gray circles) were stimulated with either DMSO or the tumor neoantigens M86, Adpgk and p15E. Responding CD8⁺ T cells were identified by flow cytometry for intracellular IFNγ. DMSO, dimethyl sulfoxide; IFNγ, interferon-γ.