Supplementary Table 1.
Quality control meta-data for RNA Seq analysis of ACE 2 expression
| ID | ACE 2 RPM | ACE 2 count (raw read number) | Raw read count from fastqc | Mapped read numbers | Final unique read numbers | Mapping % (mapped reads/raw reads from fastqc) | Unique reads/mapped reads (%) | Unique reads/raw reads (%) | Condition |
|---|---|---|---|---|---|---|---|---|---|
| S1 | 11.88 | 1,166 | 101,465,082 | 98,099,054 | 82,972,027 | 96.682575 | 84.5798442 | 81.77397127 | DKD |
| S10 | 32.35 | 3,808 | 126,566,284 | 117,684,063 | 98,667,865 | 92.98215866 | 83.8413142 | 77.95746377 | DKD |
| S11 | 3.36 | 642 | 188,577,850 | 190,596,410 | 159,468,358 | 101.070412 | 83.6680806 | 84.56367384 | DKD |
| S12 | 5.36 | 520 | 114,244,968 | 96,992,737 | 66,805,465 | 84.89891388 | 68.8767706 | 58.47563019 | DKD |
| S13 | 6.21 | 808 | 175,045,896 | 129,964,897 | 97,959,487 | 74.24618341 | 75.3738042 | 55.96217291 | DKD |
| S14 | 6.29 | 819 | 137,316,374 | 130,057,555 | 99,596,713 | 94.71379939 | 76.5789523 | 72.53083525 | Normal Control |
| S15 | 19.77 | 2,454 | 125,973,470 | 124,069,715 | 106,369,186 | 98.48876513 | 85.7334008 | 84.43776773 | DKD |
| S17 | 2.98 | 338 | 114,739,886 | 113,279,463 | 92,896,430 | 98.72718803 | 82.0064181 | 80.96263055 | Normal Control |
| S18 | 6.32 | 797 | 131,967,904 | 125,958,052 | 98,037,370 | 95.4459745 | 77.8333488 | 74.28879828 | Normal Control |
| S19 | 18.67 | 2,234 | 125,828,736 | 119,626,461 | 92,277,030 | 95.07085965 | 77.137641 | 73.33541839 | DKD |
| S2 | 32.65 | 3,358 | 107,149,218 | 102,835,831 | 81,401,804 | 95.97441112 | 79.157044 | 75.97050685 | DKD |
| S20 | 14.05 | 1,630 | 121,341,514 | 116,008,433 | 94,539,795 | 95.6048999 | 81.4938988 | 77.91216038 | DKD |
| S21 | 10.46 | 1,168 | 114,541,108 | 111,565,723 | 87,783,595 | 97.4023431 | 78.6833022 | 76.63937999 | DKD |
| S23 | 13.16 | 1,886 | 153,494,604 | 143,258,910 | 123,152,446 | 93.33156102 | 85.964947 | 80.23242693 | DKD |
| S24 | 7.58 | 1,263 | 176,090,214 | 166,535,504 | 132,321,427 | 94.57396877 | 79.4553857 | 75.14411164 | DKD |
| S26 | 5.13 | 595 | 121,143,542 | 115,801,424 | 96,940,374 | 95.59025771 | 83.7125923 | 80.02108276 | DKD |
| S28 | 13.28 | 1,584 | 127,927,852 | 119,193,003 | 93,410,716 | 93.1720506 | 78.3692949 | 73.01827908 | Normal Control |
| S29 | 8.96 | 1,094 | 124,756,622 | 122,075,555 | 98,496,727 | 97.85096217 | 80.6850536 | 78.95110129 | Normal Control |
| S3 | 19.64 | 1,911 | 108,050,092 | 97,254,218 | 73,226,930 | 90.0084546 | 75.2943487 | 67.77127964 | DKD |
| S30 | 4.35 | 469 | 116,072,918 | 107,717,085 | 72,359,389 | 92.80122087 | 67.1754058 | 62.33959674 | Normal Control |
| S31 | 5.81 | 599 | 126,222,334 | 102,977,820 | 76,401,402 | 81.58446824 | 74.192095 | 60.52922615 | DKD |
| S32 | 37.61 | 3,643 | 103,394,492 | 96,860,281 | 85,553,782 | 93.68031036 | 88.3270017 | 82.74500928 | DKD |
| S33 | 9.95 | 1,149 | 124,113,112 | 115,461,620 | 101,243,718 | 93.02934891 | 87.6860363 | 81.57374863 | DKD |
| S34 | 6.1 | 723 | 128,575,262 | 118,359,206 | 93,275,371 | 92.05441557 | 78.8070266 | 72.5453478 | Normal Control |
| S35 | 13.45 | 1,796 | 136,947,722 | 133,454,499 | 102,748,768 | 97.44922884 | 76.9916105 | 75.02773065 | Normal Control |
| S36 | 12.31 | 1,252 | 106,491,158 | 101,699,951 | 76,515,285 | 95.50084055 | 75.2363047 | 71.85130337 | DKD |
| S38 | 6.34 | 660 | 108,149,060 | 104,020,509 | 86,927,172 | 96.1825364 | 83.5673396 | 80.37718682 | DKD |
| S4 | 9.09 | 892 | 105,342,072 | 98,043,413 | 83,559,528 | 93.07146816 | 85.2270698 | 79.3220851 | DKD |
| S40 | 22.44 | 2,083 | 102,534,852 | 92,791,329 | 80,415,714 | 90.49735499 | 86.6629618 | 78.42768818 | DKD |
| S43 | 5.82 | 717 | 129,310,302 | 123,119,602 | 91,843,518 | 95.21252375 | 74.5969907 | 71.02567744 | Normal Control |
| S47 | 10.53 | 1,301 | 125,599,084 | 123,536,436 | 97,933,170 | 98.35775235 | 79.2747251 | 77.97283776 | Normal Control |
| S48 | 11.09 | 1,358 | 129,861,432 | 122,361,656 | 88,440,726 | 94.22478569 | 72.2781375 | 68.10392018 | Normal Control |
| S49 | 19.56 | 2,664 | 140,716,858 | 136,163,725 | 113,595,350 | 96.76433011 | 83.4255599 | 80.72618421 | DKD |
| S5 | 20.66 | 2,525 | 129,277,952 | 122,208,257 | 92,915,798 | 94.53139929 | 76.0307039 | 71.87288827 | DKD |
| S50 | 8.47 | 1,178 | 145,194,772 | 138,993,857 | 122,500,834 | 95.72924361 | 88.1339914 | 84.37000335 | DKD |
| S51 | 8.62 | 1,009 | 121,066,116 | 117,037,226 | 103,152,225 | 96.67215722 | 88.1362525 | 85.20321656 | DKD |
| S52 | 5.79 | 664 | 119,156,404 | 114,491,282 | 100,216,841 | 96.08487514 | 87.53229 | 84.10529156 | DKD |
| S53 | 10.78 | 1,439 | 140,139,922 | 133,387,449 | 117,250,406 | 95.18162069 | 87.9021279 | 83.66666994 | DKD |
| S54 | 12.34 | 1,677 | 138,605,802 | 135,790,629 | 116,695,942 | 97.96893567 | 85.9381408 | 84.19268192 | DKD |
| S55 | 10.68 | 1,134 | 108,659,548 | 106,132,291 | 85,310,691 | 97.67415101 | 80.3814656 | 78.51191411 | DKD |
| S56 | 15.24 | 1,993 | 128,886,930 | 130,769,149 | 94,009,761 | 101.4603645 | 71.8898622 | 72.93971623 | DKD |
| S57 | 20.15 | 3,081 | 151,341,932 | 152,896,130 | 126,284,590 | 101.0269447 | 82.5950206 | 83.44322577 | DKD |
| S58 | 13.2 | 1,746 | 131,157,148 | 132,245,790 | 91,054,983 | 100.8300287 | 68.8528406 | 69.42433896 | DKD |
| S59 | 6.19 | 1,056 | 170,223,200 | 170,469,398 | 120,482,742 | 100.1446325 | 70.6770502 | 70.77927216 | DKD |
| S60 | 14.12 | 1,667 | 121,045,880 | 117,986,535 | 89,261,896 | 97.47257404 | 75.6543075 | 73.74220089 | DKD |
| S61 | 9.1 | 1,198 | 135,099,374 | 131,562,506 | 105,719,709 | 97.38202488 | 80.3570198 | 78.25329302 | DKD |
| S62 | 2.64 | 366 | 135,216,050 | 138,598,101 | 107,703,422 | 102.5012201 | 77.7091614 | 79.65283855 | DKD |
| S63 | 5.95 | 786 | 133,282,782 | 132,057,096 | 107,361,864 | 99.08038684 | 81.2995797 | 80.55193806 | DKD |
| S64 | 10.73 | 1,520 | 146,982,650 | 141,617,337 | 121,123,920 | 96.34969638 | 85.5290197 | 82.40695075 | DKD |
| S65 | 23.04 | 2,726 | 121,592,562 | 118,315,222 | 97,936,482 | 97.30465421 | 82.7758934 | 80.54479681 | DKD |
| S66 | 2.11 | 371 | 173,153,814 | 175,225,951 | 137,535,251 | 101.1967031 | 78.4902295 | 79.42952443 | DKD |
| S67 | 17 | 2,612 | 147,157,566 | 153,576,126 | 115,308,540 | 104.3616921 | 75.0823341 | 78.35719436 | DKD |
| S68 | 19.1 | 2,359 | 124,788,568 | 123,450,100 | 102,452,239 | 98.92741136 | 82.9908109 | 82.10066086 | DKD |
| S69 | 11.19 | 1,781 | 160,522,214 | 159,145,496 | 135,084,192 | 99.14235048 | 84.8809394 | 84.15295842 | DKD |
| S7 | 3.72 | 550 | 146,984,322 | 147,547,677 | 120,541,988 | 100.3832756 | 81.6969745 | 82.01009901 | Normal Control |
| S70 | 12.69 | 1,689 | 133,615,280 | 133,031,354 | 107,608,627 | 99.56297962 | 80.8896728 | 80.53616847 | DKD |
| S71 | 14.18 | 2,045 | 140,677,196 | 144,153,124 | 111,566,950 | 102.4708539 | 77.39475 | 79.30706125 | DKD |
| S72 | 6.01 | 1,634 | 281,300,140 | 271,680,124 | 207,399,305 | 96.58015954 | 76.3395209 | 73.72883106 | DKD |
| S73 | 4.77 | 483 | 123,833,718 | 101,246,379 | 61,657,133 | 81.75994441 | 60.8981117 | 49.79026229 | DKD |
| S8 | 24.02 | 2,450 | 103,952,142 | 101,965,133 | 79,035,117 | 98.08853482 | 77.511905 | 76.0302919 | DKD |
| S9 | 13.9 | 1,671 | 130,740,442 | 120,131,959 | 109,764,113 | 91.88584432 | 91.3696188 | 83.95574569 | DKD |
ACE 2, angiotensin-converting enzyme 2; DKD, diabetic kidney disease; ID, identification; RPM, reads per million read.