Fig. 3.

Effects of miR-495-3p mimic on the proliferation, apoptosis, migration, and invasion of PCa cells. a QRT-PCR was conducted for the evaluation of the expression of miR-495-3p in both 22Rv1 and LNCaP cells. a–j 22Rv1 and LNCaP cells were transfected with miR-NC or miR-495-3p. b, c The proliferation capacity of 22Rv1 and LNCaP cells was determined with MTT assay. d The apoptotic rate of 22Rv1 and LNCaP cells was assessed using flow cytometry assay. e, f The migration and invasion capacities of 22Rv1 and LNCaP cells were detected using transwell assay. g–j The levels of Bax, Cleaved-casp-3, and Bcl-2 in 22Rv1 and LNCaP cells were measured by western blot analysis. The experiments were performed in triplicate. ***P < 0.001