Fig. 5.

Reduced miR-495-3p expression mitigated NORAD knockdown-mediated influence on cell proliferation, apoptosis, migration and invasion in PCa cells. a QRT-PCR was conducted for the evaluation of the expression of miR-495-3p in 22Rv1 and LNCaP cells. a–j 22Rv1 and LNCaP cells were transfected with si-NORAD, si-NC, si-NORAD + anti-miR-NC, or si-NORAD + anti-miR-495-3p. b, c The proliferation of 22Rv1 and LNCaP cells was analyzed with MTT assay. d Flow cytometry assay was executed for the assessment of the apoptosis of 22Rv1 and LNCaP cells. e, f Teanswell assay was carried out for the detection of cell migration and invasion in 22Rv1 and LNCaP cells. g–j The levels of Bax, Cleaved-casp-3, and Bcl-2 in 22Rv1 and LNCaP cells were assessed with western blot analysis. The experiments were performed in triplicate. *P < 0.05, **P < 0.01, and ***P < 0.001