Fig. 4.

LINC00667 enhances YY1 expression by sequestering miR-449b-5p in CRC cells. a The putative binding sites of miR-449b-5p in LINC00667 or YY1 3′-UTR. b Luciferase reporter assay was conducted in two CRC cells to determine the interaction between miR-449b-5p and LINC00667. N = 3 in each group. c The luciferase activity of YY1-WT or YY1-Mut vector was measured after co-transfected with NC mimics or miR-449b-5p mimics. N = 3 in each group. d Ago2-RIP assay was performed in two CRC cells to identify the enrichment of LINC00667, miR-449b-5p and YY1 in the immunoprecipitates conjugated to Ago2 antibody. N = 3 in each group. e MiR-449b-5p expression was decreased after transfected with miR-449b-5p inhibitor for 48 h. NC inhibitor was used as negative control. N = 3 in each group. f The luciferase intensity of YY1-WT or YY1-Mut reporter plasmid was detected in cells co-transfected with shNC, shLINC00667#2 or shLINC00667#2 + miR-449b-5p inhibitor. N = 3 in each group. g The mRNA and protein levels of YY1 were measured in two CRC cells transfected with shNC, shLINC00667#2 or sh-LINC00667#2 + miR-449b-5p inhibitor. N = 3 in each group. ^*P < 0.05, ^***P < 0.001 compared to the control group