Skip to main content
. Author manuscript; available in PMC: 2021 Jul 16.
Published in final edited form as: Cell Chem Biol. 2020 May 14;27(7):866–876.e8. doi: 10.1016/j.chembiol.2020.04.008

Figure 6. Design and Development of HDAC6 Degraders Based on Pan HDAC Inhibitor and CRBN Ligands.

Figure 6.

(A) Chemical structures of HDAC degraders 6g, 6i and 9d. (B) and (C) Western blot analysis of proteins expression of MM1S cells treated with degraders or vehicle for 6 h. (D) In-cell ELISA analysis of HDAC6 expression undertreatment of degraders. MM1S cells were treated with degraders or vehicle (6 h) and then analyzed by Western blot or in-cell ELISA. Bar graphs were generated as means of protein (IKZF1 or IKZF3) relative expression (n = 3) with ± SD as the error bar. Dose response were nonlinear fitted by [Inhibitor] vs. response (three parameters) and fitted curve was generated in GraphPad Prism.