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. 2020 Jul 18;11(7):547. doi: 10.1038/s41419-020-02753-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative micrographs (left) and quantification (right) of colony formation assays for SKBR3 and BT474 cell lines with or without overexpression of NCAPG under treatment with trastuzumab (10 μg/mL). b Cell viability measured by MTT assay. c Flow cytometry analysis of apoptosis by Annexin V-FITC/PI staining (left) and histograms (right) indicating the percentage of Annexin V+ cells. d Representative images (left) and quantification (right) of fluorescence micrographs of TUNEL-stained cells in the indicated cells treated with trastuzumab (10 μg/mL). Error bar represents the mean ± SD of three independent experiments. *P < 0.05.