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. 2020 Jul 18;10:128. doi: 10.1186/s13568-020-01064-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Genotoxicity of CPA, PPA or 4-OH-CPA^UPO on HepG2 and MCF-7 cells. Cells were treated with different concentrations of the respective compounds for 24 h. γH2AX foci formation was determined using indirect immunofluorescence (pink). Nuclei were stained with DAPI (blue). Representative images of (a) HepG2 and (b) MCF-7 nuclei are shown as obtained with Olympus IX81 fluorescence microscope and 100 × objective (scale bar 5 µm)