FIG. 2.

Adoptive transfer of the immunized AAD mouse splenocytes prevents and eradicates large HepG2 tumors in NSG mice. (A) Approximately 11% of the prime-boosted AAD mouse splenocytes produced IFNγ in response to AFP₁₅₈ peptide. (B) In the preventive model, 15 million total splenocytes of the naive or immunized mice were injected into NSG mice, which was followed by HepG2 tumor cell challenge 2 days later. The tumor growth curve is shown. (C) In the therapeutic model, NSG mice were injected with 15 million total splenocytes of the immunized mice when HepG2 tumor size reached 2 cm in diameter. Shown is the tumor volume curve. The experiment was done twice with similar data. Abbreviation: ACT, Adoptive cell transfer.