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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Tissue Eng Regen Med. 2019 Dec 21;14(3):464–474. doi: 10.1002/term.3007

Figure 3.

Figure 3.

Phenamil and HAGF scaffold regulates adipogenesis in C3H10T1/2 cells. (A-B) Oil Red O staining (10X) showed increasing lipid content in adipogenic medium on 2D wells at 0 micromolar (uM) (A) and 20 uM (B). Lipid content sequentially increased as phenamil concentration increased (C) on 2D plate. In 3D HAGF scaffold, adipogenic gene expressions of (D) peroxisome proliferator-activated receptor gamma (PPARg), (E) fatty acid-binding protein (Fabp4), and (F) lipoprotein lipase (LPL) reduced after 14d cell culture in adipogenic medium under phenamil treatment at 20 uM (n=3). uM = μM.*p<0.05. HAGF: hydroxyapatite-coated nanofibrous gelatin scaffold. GF: nanofibrous gelatin scaffold. GF-Phe: nanofibrous gelatin scaffold with phenamil treatment. HAGF-Phe: hydroxyapatite-coated nanofibrous gelatin scaffold with phenamil treatment.