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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Tissue Eng Regen Med. 2019 Dec 21;14(3):464–474. doi: 10.1002/term.3007

Figure 4.

Figure 4.

Phenamil modulates MC3T3-E1 osteoblastic differentiation. (A) Alkaline phosphatase (ALP) staining showed increased activity in MC3T3-E1 cells with phenamil addition (purple color) at concentrations of 0 to 20 micromolar (uM). (B) Quantitative ALP activity exhibited by phenamil concentration-dependency over 7 d culture (n=3). Gene expression of MC3T3-E1 cells on different scaffold were observed on day 7 (yellow) and day 14 (blue). Hydroxyapatite and phenamil affected genome expression for (C,D) RUNX2, (E,F) OCN and (G,H) BSP after 7 d or 14 d (n=3). *p<0.05. RUNX2: Runt-related transcription factor. OCN: osteocalcin. BSP: bone sialoprotein.