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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Tissue Eng Regen Med. 2019 Dec 21;14(3):464–474. doi: 10.1002/term.3007

Figure 5.

Figure 5.

Phenamil rescued LPS-inhibited osteogenic activity. (A) ALP staining and quantitative data of MC3T3-E1 cells showed phenamil-rescued LPS-induced ALP (purple color). (B) Alizarin Red S (10X) staining showed increasing calcium content when treated with BMP2 or phenamil for 28 d. No calcium content detected in LPS. Quantitative results of calcium content when treated with BMP2 and phenamil for 28 d (n=3). (C) HAGF scaffold increased MC3T3-E1 calcium mineralization before and after stimulation with BMP2 and phenamil after 28 d cell culture in osteogenic medium (n=3). BMP2 (200 ng/mL). Phenamil (Phe, 20 mM). L10 (10 ng/mL LPS). L2 (2 mg/mL LPS). *p<0.05. LPS: lipopolysaccharide. BMP2: bone morphogenetic protein 2.