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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: J Immunol. 2020 Jun 26;205(3):830–841. doi: 10.4049/jimmunol.2000322

Figure 1. TRAF3 restrains BCR’s capacity to induce AID and CSR.

Figure 1.

(A) Flow data showing IgG1 and IgE CSR kinetics induced by anti-IgM/IL-4 in indicated B cells. (B) Quantification of IgG1+ B cell percentage from triplicates of one representative experiment. (C) Western blot data showing AID protein expression induced by anti-IgM/IL-4 in indicated B cells. GAPDH as loading control. d0: day0 (unstimulated), d3: day3 (3 days after stimulation). (D) Flow data showing day4 IgG1 and IgE CSR induced by anti-IgD/IL-4. (E) Western blot data showing AID protein expression induced by anti-IgD/IL-4 in indicated B cells. (F) Flow data showing day4 IgG1 and IgE CSR induced by anti-IgM/IL-4 or HEL/IL-4 in indicated B cells. (G) Quantification of IgG1+ B cell percentage from triplicates of one representative experiment. (H) Western blot showing AID protein expression induced by HEL/IL-4 in indicated B cells. TRAF3-cKO, CD19Cre-Traf3f/f. Data are representative of 3-6 independently repeated experiments.