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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: J Immunol. 2020 Jun 15;205(3):760–766. doi: 10.4049/jimmunol.1901423

Figure 2. Lm-induced pro-inflammatory cytokine production in P2X5-deficient mice.

Figure 2.

(a) IL-12p40 and IFN-γ were measured from collected serum at days 0, 1 and 2 post-Lm infection by ELISA. (b) Splenocytes from mice 0, 1 and 2 days post-Lm infection were cultured with or without HKLM stimulation for 4 hr in the presence of BFA and stained for intracellular TNF-α with surface staining for CD4, CD8a, and CD11b. Cell numbers are gated from non-CD4, CD8 and CD11bint TNF-αhi lymphocytes/monocytes. Data are representative of at least two independent experiments. (**p < 0.01)