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. 2020 Jul 7;13:6617–6628. doi: 10.2147/OTT.S249353

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© 2020 Zou et al.

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Characterization and growth of HCT116-CSCs. (A) HCT116-CSCs culture (× 200). 1, HCT116-CSCs on day 5 after incubation; A2, HCT116-CSCs on day 5 after successive 6 passages of incubation; (B) Flow cytometry detects the percentages of CD133⁺CD44⁺ cells in parental HCT116 cells and HCT116-CSCs; (C) Colony-formation assay measures the colony formation of parental HCT116 cells and HCT116-CSCs. *P < 0.05.