Table 3.

Quantitative Immunoblotting Results of Involucrin, E-Cadherin (80 and 120 kDa), PCNA and MMP-9 in HaCaT Cells Treated with Unmodified and Polyphenol-Modified Au@AgNPs. Cells Were Treated at 5 µg/Ml with Citrate, Unmodified Au@AgNPs, GAL-Au@AgNPs, TAN-Au@AgNPs, ECG-Au@AgNPs, RES-Au@AgNPs and PRO-Au@AgNPs for 24h and Subjected to Western Blotting Assay. The Results are Expressed as Density Measurements from 3 Experiments ± SEM

	PCNA	E-Cadherin 120	E-Cadherin 80 kDa	Involucrin	MMP-9
Control	0.38 ± 065	0.31 ± 0.051	0.23 ± 0.038	0.52 ± 0.1	0.29 ± 0.05
Ag@AuNPs	0.41 ± 0.046	0.35 ± 0.064	0.31 ± 0.09	0.68 ± 0.1	0.05 ± 00.1**
GAL-Ag@AuNPs	1.49 ± 0.15*	0.77 ± 0.02*	0.032 ± 0.001**	0.74 ± 0.1	0.74 ± 0.04**
GAL	1.67 ± 0.3*	1.23 ± 0.43**	0.057 ± 0.004*	0.55 ± 0.21	0.55 ± 0.03*
TAN-Ag@AuNPs	0.43 ± 0.088	0.35 ± 0.092	0.32 ± 0.09	0.66 ± 0.11	0.39 ± 0.01
TAN	0.49 ± 0.08	0.29 ± 0.035	0.3 ± 0.06	0.57 ± 0.09	0.45 ± 0.012
ECG-Ag@AuNPs	1.5 ± 0.3*	0.79 ± 0.029*	0.017 ± 0.001**	0.85 ± 0.06	0.97 ± 0.11*
EGG	1.7 ± 0.45**	0.88 ± 0.124*	0.018 ± 0.001**	1.05 ± 0.13**	1.05 ± 0.11*
PRO-Ag@AuNPs	1.27 ± 0.3*	0.75 ± 0.1*	0.14 ± 0.09*	0.75 ± 0.07	0.75 ± 0.089*
PRO	1.62 ± 0.4**	0.7 ± 0.02*	0.09 ± 0.001**	0.62 ± 0.15	0.62 ± 0.1*
RES-Ag@AuNPs	1.1 ± 0.1*	0.9 ± 0.092*	0.11 ± 0.09*	0.52 ± 0.095	0.52 ± 0.1*
RES	1.3 ± 0.093*	1 ± 0.126**	0.13 ± 0.02*	0.36 ± 0.1	0.36 ± 0.08

Note: *Represents significant differences with p ≤ 0.05, while **means p ≤ 0.01.

Abbreviations: TAN, tannic acid modified; GAL, gallic acid modified; ECG, epicatechin gallate modified; RES, resveratrol modified and PRO, procyanidin modified; PCNA, proliferating cell nuclear antigen; MMP-9, matrix metalloproteinase 9; S.E.M., standard error of mean.