Fig. 4.

Spontaneous locomotor activity of Rpt2 knockdown flies. Actograms are shown on top of each column; quantization of the corresponding actograms and periods are shown in the lower panels. Panels A, B and C show actograms for days 3 to 5, 8 to 10, and 15 to 17 respectively. Hyperactivity in RNAi-Rpt2 (experimental) flies (red) begins to show only after the fifth day of aging. In every 3 day shown, experimental animals (red) accumulated significantly more activity than controls (black). D) Comparison of accumulated activity during the light and dark periods for days 3 and 4 was not significantly different between the control and RNAi-Rpt2, but at day 5 RNAi-Rpt2 group showed an increase in activity that is statistically different. Panels E (light period) and F (dark period) show accumulated activity for days 3 to 5, hyperactivity in RNAi-Rpt2 (experimental) flies (red) begins to show only after the fourth day of aging. G) Accumulated activity for days 8 to 10 quantified during the light and dark periods. Increased activity in RNAi-Rpt2 flies is significantly different from controls. Panels H (light period) and I (dark period) show accumulated activity for days 8 to 10; RNAi-Rpt2 flies have more accumulated activity than controls only in the light period. J) Accumulated activity for days 15 to 17 quantified during the light and dark periods. RNAi-Rpt2 flies have more accumulated activity than controls. Panels K (light period) and L (dark period) show accumulated activity for days 15 to 17 quantified during the light and dark periods respectively; in both periods RNAi-Rpt2 has more accumulated activity that is significantly different than controls. Significance was tested for each independent period using unpaired t-test, n ≥ 23, Bar graph = mean with SEM (* = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.001, **** = P ≤ 0.0001).